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PCR validation of key genes not affected by the interaction between Tat expression and Meth during sensitization. The primers for (A) SIRT1; (B) amyloid precursor protein (APP); (C) NOS2; and (D) PPARa were purchased from Qiagen, and the expression was normalized by the average of 2 housekeeping genes, 18S and GAPDH. * p < 0.05 in ANOVA followed by Bonferroni’s post hoc test between assigned comparisons.
