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. 2020 Apr 15;12(4):447. doi: 10.3390/v12040447

Figure 8.

Figure 8

Mutation of tRNA binding sites permits recognition of Ub-modified Gag. The yeast 2-hybrid assay was used to measure the relative binding for p6 plus Tsg101 wild-type or mutants and p6-Ub plus Tsg101 wild-type or mutants. The figure shows the average for transformants representing two or three isolates for each mutation. The asterisks indicate the mutants that were different from Tsg101 WT by Student’s t-test (* p < 0.05). For each assay, wild-type controls (n = 3) were run in parallel with experimental samples and were used to normalize values. Controls also included Gag p6 L7TAP plus Tsg101 wild-type (negative control) and Gag p6 2xPTAP plus Tsg101 wild-type (positive control) for each assay.