Figure 1.

Co-infection of tobacco curly shoot virus (TbCSV) and its betasatellite (TbCSB) in N. benthamiana plants. (A) Leaf curling symptoms in a N. benthamiana plant co-inoculated with TbCSV and TbCSB. The plant inoculated with Agrobacterium cells without viral constructs (Mock) was used as control. The plants were photographed at 7 days post agro-infiltration (dpai). (B) Analysis of TbCSV and TbCSB DNA accumulations through PCR. The expression of N. benthamiana Ubiquitin C gene (UBC) was used as an internal control. (C) Analysis of nbe-miR1919c-5p expression in the mock- and the TbCSV/TbCSB-inoculated plants through qRT-PCR. Statistical differences between the treatments were determined by the Student’s t-test, * p < 0.05.