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. 2020 Apr 1;12(4):392. doi: 10.3390/v12040392

Figure 5.

Figure 5

Analyses of the expression of nbe-miR1919c-5p target genes (Niben001 and Niben007) through qRT-PCR. (A) At seven days post TbCSV/TbCSB co-inoculation, the assayed plants were sampled and analyzed for the expression of Niben001 and Niben007 through qRT-PCR. N. benthamiana plants inoculated with Agrobacterium cells without virus infectious clones (Mock) were used as controls. (B) Detection of Niben001 and Niben007 expression in the PVX-inoculated or the PVX-M1919-inoculated plants through qRT-PCR. (C) Detection of Niben001 and Niben007 expressions in the TRV-inoculated or the TRV-M1919-inoculated plants through qRT-PCR. (D) Detection of Niben001 and Niben007 expressions in the PVX+TbCSV/TbCSB-inoculated or the PVX-M1919+TbCSV/TbCSB-inoculated plants through qRT-PCR. (E) Detection of Niben001 and Niben007 expression in the TRV+TbCSV/TbCSB-inoculated or the TRV-M1919+TbCSV/TbCSB-inoculated plants through qRT-PCR. (F) Detection of Niben001 and Niben007 expression in the pGD-OV1919-inoculated or the pGD-GFP-inoculated plants through qRT-PCR. (G) Detection of Niben001 and Niben007 expressions in the pGD-OV1919+TbCSV/TbCSB-inoculated or the pGD-GFP-M1919+TbCSV/TbCSB-inoculated plants through qRT-PCR at 3 dpi. (H) Detection of Niben001 and Niben007 expressions in the pGD-OV1919+TbCSV/TbCSB-inoculated or the pGD-GFP-M1919+TbCSV/TbCSB-inoculated plants through qRT-PCR at 5 dpi. * p < 0.05, ** p < 0.01; determined by the Student’s t-test.