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The yeast two-hybrid protein-protein interaction of CSFV protein E2 or E2-∆SERTAD (T149A, Y325h and H335R) coupled to the Gal4 binding domain with SERTAD1 or HPRT coupled to the Gal4 activation domain. Spots of strains (10 uL), expressing the indicated constructs were spotted on (A) selective media SD-Ade/His/Leu/Trp or (B) non-selective media SD-Leu/Trp.
