Figure 5.

Validation of the differentially expressed ISG induced transcripts by RT-qPCR by SYBR Green chemistry. The PBMCs of goat and cattle were infected with PPRV at 1 MOI and RT-qPCR was performed on the 24 h PI, using the primers listed in Table 1. PPRV infection resulted in significantly increased levels of IFIT1, IFIT2, IFIT3, DDX58, DHX58, IRF7, MX1, MX2, IGS15, RSAD2, IFI44 and IFI6 in cattle, while PDE12 significantly increased in goat PBMC upon PPRV infection. (* indicates p < 0.05 determined by paired t-test and results of n = 5 samples).