Table 5.
Effect of itaconic acid on growth and ICA production by Y. lipolytica VKM Y-2373.
| Parameter | Itaconic Acid (mM) | ||||
|---|---|---|---|---|---|
| 0 | 15 | 30 | 60 | 100 | |
| Biomass (g/L) | 18.30 ± 3.10 | 19.25 ± 2.10 | 17.70 ± 1.75 | 16.45 ± 1.10 | 11.45 ± 3.20 |
| ICA (g/L) | 80.12 ± 2.00 | 90.2 ± 1.10 | 84.1 ± 2.00 | 68.75 ± 2.00 | 40.0 ± 3.65 |
| CA (g/L) | 41.00 ± 3.12 | 29.6 ± 1.10 | 27.8 ± 1.70 | 20.8 ± 2.70 | 12.5 ± 0.65 |
| ICA/CA ratio | 2:1 | 3:1 | 3:1 | 3.3:1 | 3.2:1 |
| Total amount of ICA (P) (g) | 597.60 | 648.00 | 576.00 | 511.20 | 273.60 |
| Glycerol + Fatty acids consumed (S) (g) | 602.05 | 584.43 | 586.53 | 620.65 | 588.86 |
| Y (g/g) | 0.94 | 1.11 | 0.98 | 0.82 | 0.46 |
| Q (g/L·h) | 0.83 | 0.90 | 0.80 | 0.71 | 0.38 |
| Enzyme activity (U/mg protein) | |||||
| Citrate synthase | 2.910 | 2.800 | 2.750 | 2.350 | 2.10 |
| Aconitate hydratase | 0.805 | 0.830 | 0.730 | 0.730 | 0.630 |
| NAD-ICDH | 0.060 | 0.064 | 0.031 | 0.022 | 0.020 |
| Isocitrate lyase | 0.081 | 0.010 | 0.008 | 0.002 | 0.002 |