Table 2.

Primer sequences used to amplify each species.

Target Specie	Primer Sequence (5’–3’)	Gene Target	Amplicon Length (bp)	Reference
C. difficile	F-GTTAGCATCCGTATTAGCAGGTGC R-ACAGCTATGGGTGCGAATGG	tcdA	135	This study
E. coli	F-TCCTGGATTGAGGTGCTTTATC R-CTACGGAGACCTGGGTAATTCC	yedN	142	Elkins et al. [19]
V. parahaemolyticus	F-ACTGGATTTCGCTTTGCCCTCAATGA R-GTTCTGAGTTCGATAACCTCTTGTGTGGATTAAG	tlh	146	This study
B. cereus	F-GAAAAGTACAGTGGCAAAAGTTGTTGCG R-CGCTAACTCTTGCTGACGACGT	cmk	313	This study