FIGURE 1.

dnmA and fisA genes are required for normal growth and asexual development. (A) Conidia (1x10⁴) from strains TRV1 (WT), CVG1 (ΔdnmA), CVG2 (ΔfisA), and CVG3 (ΔdnmA ΔfisA) were inoculated on supplemented MM plates and incubated at 37°C for 3 (upper panel) or 6 (middle panel) days (D). Lower panels show enlargements from 6-day colony borders. (B) Total conidiospores per colony were harvested, counted and the count divided by colony area to determine number of conidia per square centimeter. Black and gray bars represent 3 D and 6 D spore numbers, respectively. Standard deviation from three independent experiments is indicated. Data analyzed by one-way ANOVA, following Tukey’s test (^∗p< 0.05). Asterisks indicate significant differences with respect to the WT strain. (C) Conidiophores from the indicated strains were obtained from 3 and 6-day colonies and observed under the microscope. White arrowheads point to conidiophore heads. Black bars = 20 μm.