FIGURE 10.
Pretreatment with Mito TEMPO prevents MitoSOX but not Mitotracker staining of ΔdnmA and ΔfisA mitochondria. Mycelia from strains 1155 (WT), TVG1 (ΔdnmA), and TVG2 (ΔfisA) grown for 14 h in supplemented glucose minimal medium were incubated with Mito TEMPO for two h at 37°C and then stained with either MitoSOX or Mitotracker, as indicated in section “Materials and Methods,” and then observed by using confocal microscopy.