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. 2020 May 4;11:837. doi: 10.3389/fmicb.2020.00837

FIGURE 10.

FIGURE 10

Pretreatment with Mito TEMPO prevents MitoSOX but not Mitotracker staining of ΔdnmA and ΔfisA mitochondria. Mycelia from strains 1155 (WT), TVG1 (ΔdnmA), and TVG2 (ΔfisA) grown for 14 h in supplemented glucose minimal medium were incubated with Mito TEMPO for two h at 37°C and then stained with either MitoSOX or Mitotracker, as indicated in section “Materials and Methods,” and then observed by using confocal microscopy.