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The lack of mitochondrial fission results in decreased in vivo respiration. Conidia from strains TRV1 (WT), CVG1 (ΔdnmA), CVG2 (ΔfisA), and CVG3 (ΔdnmA ΔfisA) were incubated in supplemented glucose-MM for 4 h, washed three times with ice-cold MM without glucose and maintained on ice until used for respiration measurements. Respiration was determined using an Oroboros high-resolution respirometer, as indicated in section “Materials and Methods.”
