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. 2020 May 4;11:837. doi: 10.3389/fmicb.2020.00837

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Copyright © 2020 Garrido-Bazán, Pardo and Aguirre.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The lack of mitochondrial fission results in increased mitochondrial ROS production. (A) Mycelia from strains A1155 (WT), TVG1 (ΔdnmA), and TVG2 (ΔfisA) grown for 14 h in supplemented glucose minimal medium were treated with MitoSOX as indicated in Materials and Methods and then observed by using confocal microscopy. (B) Mycelia from strain A1155 (WT) grown for 14 h in supplemented 1% ethanol minimal medium was treated with MitoSOX as before and observed by using confocal microscopy. BF, bright field. White bars = 5 μm.