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. 2020 May 8;11:357. doi: 10.3389/fgene.2020.00357

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Copyright © 2020 Deng, Li, Wang, Geng, Cai, Tang, Wang, Yu, Li and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Downregulation of miR-135a-5p rescues the inhibition of colorectal cancer (CRC) cell proliferation induced by depletion of circ_0001946. (A) The predicted binding regions of circ_0001946 on miR-135a-5p (upper panel) and the relative luciferase activity in 293T cells after co-transfection with either pmirGLO-Circ_0001946-WT or pmirGLO-Circ_0001946-MUT, along with either miR-135a-5p specific mimics or negative control (NC). (B) The expression of miR-135a-5p in circ_0001946-downregulated LoVo cells and the successful downregulation of miR-135a-5p in LoVo cells after transfection with circ_0001946-specific siRNAs using miR-135a-5p inhibitors. (C) The growth of circ_0001946-downregulated LoVo cells, with or without depletion of miR-135a-5p, as detected by the Cell Counting Kit-8 (CCK-8) assay. All experiments were repeated at least three times. ***P < 0.001.