Figure 7.

The impact of Gent on primary RA-FLS viability and TNF-α-induced proliferation and migration. (A) Cell viability was assessed via MTT assay after a 72-h treatment with the indicated Gent concentrations. (B) Following Gent or Dex pretreatment, RA-FLS were treated using 10 ng/mL TNF-α for 72 h, after which an MTT assay was used to quantify cell proliferation. (C) RA-FLS proliferation was also assessed based on EdU (green) incorporation, with DAPI-stained nuclei shown in blue (scale bar = 50 μm). (D) Cell migration in a wound healing assay was quantified following a 48-h incubation with or without the indicated Gent and Dex doses (100 × magnification). (E, F) Quantification of the data shown in C and D. Data were presented as the mean ± SD from three independent experiments. ^##P < 0.01, ^###P < 0.001 vs. control; **P < 0.01, ***P < 0.001 vs. TNF-α group.