FIG. 7.

Dose-dependent effects of bimatoprost acid on levels of mRNA for MMPs and TIMPs in human nonpigmented ciliary epithelial cells. Cultures were exposed to 10, 100, or 1,000 μM bimatoprost acid (B) for 24 h or to no drug (control, C), and mRNA levels were quantified by quantitative polymerase chain reaction. *P < 0.05, **P < 0.01, ***P < 0.001 vs. control. Adapted from Yamada et al.⁸⁷ under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/). The superimposed black and red arrows show for comparison the maximal iris-ciliary body bimatoprost plus bimatoprost acid concentrations obtained after topical bimatoprost treatment and Bimatoprost SR administration, respectively, in a drug distribution study in beagle dogs.¹⁰² Bimatoprost SR, bimatoprost sustained-release implant; TIMP, tissue inhibitor of metalloproteinase.