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. 2020 May 8;31(9-10):575–589. doi: 10.1089/hum.2019.264

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© Adrian Westhaus et al., 2020; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons Attribution Noncommercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and the source are cited.

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Figure 1. — Experimental setup and preparation of the AAV Testing Kit mix. (A) The ITR2-CMVp-eGFP-N₆BC-WPRE-ITR2 transgene packaged in the individual AAV vectors used in the study. (B) Individual steps involved in the preparation of the AAV Testing Kit. (C) Upper panel: titration of individually produced AAVs (Step 1) and diluted AAV variants (Step 2). The dilution benchmark of 2.5 × 10¹¹ vgc/mL is indicated by the orange dotted line. Lower panel: Results of the NGS analysis of the AAV distribution in the AAV Testing Kit used in this study. The expected % contribution of individual variants (100% ÷ 30 = ∼3.33%) is indicated by the green dotted line. AAV, adeno-associated virus; N₆BC, unique 6 nucleotide barcode; NGS, next-generation sequencing.