Table 2.
Determination of molar ratios of H‐chain, L‐chain, and fhx/P25 in the fibroin secreted into cocoons of a normal breed (C108), Nd‐sD mutant, or transgenic lines of B. mori. A 490 values were obtained by ELISA. Protein samples (all in 100 µL) assayed were 10 ng for H‐chain, 50 ng for L‐chain and 125 ng for fhx/P25 from cocoons of C108 and the transgenic line L6 × 7, or 2 µg for H‐chain, 10 µg for L‐chain and 100 µg for fhx/P25 from naked‐pupa cocoons of Nd‐s D and cocoons of the transgenic line L7‐4. n = 6; ±, SD; ND, not detected.
| Breed or line | A 490 | ng/100 µg protein | pmol | Molar ratio | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| H‐chain | L‐chain | fhx/P25 | H‐chain | L‐chain | fhx/P25 | H‐chain | L‐chain | fhx/25 | H‐chain: L‐chain: fhx/P25 | |
| C108 | 0.637 | 0.354 | 0.196 | 7.75 × 104 | 5.75 × 103 | 1.12 × 103 | 2.21 × 104 | 2.23 × 104 | 3.73 × 103 | 5.92 ± 0.04 : 5.98 ± 0.05 : 1 |
| Nd‐sD | 0.401 | ND | 0.421 | 245 | ND | 3 | 70 | ND | 11.1 | 6.31 ± 0.07 : –: 1 |
| Transgenic line | ||||||||||
| L6 × 7 | 0.300 | 0.166 | 0.088 | 3.66 × 104 | 2.70 × 103 | 0.50 × 103 | 1.05 × 104 | 1.04 × 104 | 1.67 × 103 | 6.17 ± 0.05 : 6.16 ± 0.03 : 1 |
| L7‐4 | 0.625 | 0.344 | 0.186 | 761 | 5.59 | 1.06 | 218 | 215 | 35.3 | 6.18 ± 0.03 : 6.09 ± 0.04 : 1 |