Table 1.
Sample statistics, diversity, and effective population size estimates
| Method | Group | Code | N | A | H o | H e | G IS | Assignment | N e (CIs) |
|---|---|---|---|---|---|---|---|---|---|
| RAD | C. artedi | ART | 60 | 98.5% | 0.246 | 0.253 | 0.028 | 98.3%b | 1,834 (1,769–1,906) |
| C. hoyi | HOY | 21a | 94.8% | 0.219 | 0.273 | 0.196 | 92.5%b | 1,701 (1,487–1,985) | |
| C. kiyi | KIY | 25 | 93.4% | 0.217 | 0.242 | 0.103 | 100% | 2,126 (1,846–2,506) | |
| C. nigripinnis | NIG | 6 | 83.1% | 0.213 | 0.261 | 0.182 | – | – | |
| C. zenithicus | ZEN | 4 | 76.5% | 0.186 | 0.259 | 0.279 | – | – | |
| Microsatellites | C. artedi | ART | 30 | 70.0% | 0.534 | 0.607 | 0.121 | 76.7% | 91 (42–6,126) |
| C. hoyi | HOY | 21a | 65.0% | 0.629 | 0.659 | 0.046 | 61.9% | 73 (33‐Infinite) | |
| C. kiyi | KIY | 25 | 67.0% | 0.572 | 0.631 | 0.093 | 72.0% | 649 (74‐Infinite) |
N is the number of individuals successfully genotyped, A is the percentage of total sampled alleles found in each group, H o/H e is observed/expected heterozygosity, G IS is the inbreeding coefficient, Assignment is the percentage of individuals that were correctly assigned to their population of origin in a leave‐one‐out test, and N e is effective population size calculated using the LDNE method and reported with 95% confidence intervals.
Three genotyped hoyi samples are suspected to be misidentified kiyi from the RAD‐based PCA and ADMIXTURE analysis and were removed to prevent bias in estimates of diversity and N e.
The one artedi that assigned to HOY and the two hoyi that assigned to KIY appear to be hybrids. Outside of these putative hybrids, assignment to the ART and HOY groups was 100%.