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. Author manuscript; available in PMC: 2020 May 18.
Published in final edited form as: Evol Dev. 2019 May 17;21(4):188–204. doi: 10.1111/ede.12289

FIGURE 2.

FIGURE 2

Variation in lipid content of sea urchin eggs and larvae is driven by life history evolution. (a) PC analysis of normalized lipidomic mass spectrum intensities measured from egg and larva samples from each sea urchin species. PC 1 explains 77.04% of the variation and distinguishes Heliocidaris erythrogramma samples from those of the two planktotrophs. (b,c) Volcano plots illustrating differential relative abundance of 1,667 unique lipid molecules between (b) eggs and (c) larvae in H. erythrogramma (red) and Heliocidaris tuberculata (blue). Only lipids that are differentially abundant between H. erythrogramma and H. tuberculata and not differentially abundant between H. tuberculata and Lytechinus variegatus are colored to mark a high confidence set of lipids potentially associated with either life history strategy. Significantly differentially abundant lipids (n) are called as having an FC ≥ 2 and a Tukey’s honest significant difference corrected p ≤ 10%. FC: fold-change; PC: principle component