Figure 10:

Azimuthally integrated background-subtracted synchrotron small-angle X-ray scattering (SAXS) intensity curves as a function of the magnitude of the scattering vector, q. 13 μM SV40 VP1 pentamers were mixed with 0.87 μM 524nt RNA at 1:1 volume ratio, at pH 7.2 and different final NaCl concentrations, as indicated in the figure. (A) Assembly reactions at low salt concentrations (1–75 mM). The inset shows the first two minima on an expanded scale. (B) Assembly reactions at high salt concentrations (75–500 mM). At 500 mM the orange curve resembles the scattering curve of 6.5 μM SV40 VP1 pentamers in the assembly buffer and 500 mM (gray curve) or 13 μM SV40 VP1 pentamers before mixing with the RNA (in the disassembly buffer, see Methods). The curves were vertically shifted for clarity of representation.