Figure 5. RT-PCR analysis of whole lung extracts from 3-day hypoxic (HX) wild-type (C57BL6) mice, compared to sea-levloe (SL) controls, demonstrates robust augmentation of complement factor B (Cfb), modest upregulation of complement C3, and no alterations in expression of complement inhibitors/regulators (complement factor H (Cfh), and Cd55/Daf), thus shifting the balance toward local activation of the alternative complement pathway.

*P ≤ 0.05; ****P ≤ 0.0001; ns, not significant.