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. Author manuscript; available in PMC: 2020 May 18.
Published in final edited form as: Cell Rep. 2020 Apr 21;31(3):107523. doi: 10.1016/j.celrep.2020.107523

Figure 6. CytoMAP Analysis of 3D LNs Reveals Preferential Proximity of DCs with Vasculature.

Figure 6.

(A) Representative confocal image of a 500-μm-thick, Ce3D-cleared, steady-state LN slice. Main image scale bar, 200 μm; zoom-in scale bars, 50 μm.

(B) Violin plot comparing the distances of the indicated cells to the nearest blood vessels within a representative LN sample.

(C) Mean distances of cell populations to the nearest blood vessel, with each symbol representing an individual LN sample. Circles and squares representsamples from two independent experiments. Cells below the dotted line at 20 μm are considered proximal to vasculature.

(D) Percentage of proximal cells for the LN samples shown in (C).

(E) Heatmap indicating the Pearson correlation coefficients, averaged across all imaged samples, between the number of different cell or landmark object typesper 50-μm-radius neighborhood.

For this experiment, an average imaging volume of 0.93 mm3 and an average of 39,139 myeloid cells, 36,049 blood vessel objects, 98,279 spots, and 36,049 neighborhoods were analyzed per sample. Data represent four samples from two independent experiments.