KEY RESOURCES TABLE.
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| NRF2 | Abcam | Cat# ab62352; RRID: AB_944418 |
| 20-s Proteasome | UBPBio | Y2010 |
| P62 | Novus | Cat# H00008878-M01; RRID: AB_548364 |
| LC3 | Novus | Cat# NB100-2220; RRID: AB_10003146 |
| ATG5 | Cell Signaling Technology | Cat# 9980S; RRID: AB_10829153 |
| ATG7 | Cell Signaling Technology | Cat# 8558; RRID: AB_10831194 |
| ATG12 | Cell Signaling Technology | Cat # 2010s; RRID: AB_2059086 |
| STX17 | Sigma-Aldrich | Cat# HPA001204; RRID: AB_1080118 |
| FIP200 | Novus | Cat# NBP1-31583; RRID: AB_2300812 |
| PTEN | Santa Cruz Biotechnology | Cat# sc-7974; RRID: AB_628187 |
| FOXO3a | Cell Signaling | Cat# 3938; RRID: AB_2106669 |
| β-Actin | Sigma-Aldrich | Cat# A5441; RRID: AB_476744 |
| β-Tubulin | Sigma-Aldrich | Cat# T5168; RRID: AB_477579 |
| Mouse-IgG | Cell Signaling Technology | Cat# 7076; RRID: AB_330924 |
| Rabbit-IgG | Cell Signaling Technology | Cat# 7074; RRID: AB_2099233 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Bafilomycin A1 | Sigma-Aldrich | B1792; CAS RN: 88899-55-2 |
| Chloroquine | MP-Biomedicals | 93919; CAS RN: 50-63-5 |
| Protease Inhibitor Cocktail | Roche | 11836153001 |
| Bortezomib | Selleck Chemicals | S1013 |
| Critical Commercial Assays | ||
| MEGAscript T7 Transcription Kit | Thermo Fisher | AM1354 |
| MEGclear Transcription Clean Up Kit | Thermo Fisher | AM1908 |
| Wizard SV Gel PCR clean up kit | Promega | A9282 |
| Lipofectamine CRISPR Max Cas9 transfection reagent | Thermo Fisher | CMAX00003 |
| RNeasy RNA isolation kit | Qiagen | 74104 |
| QuantiTect Reverse Transcription Kit | Qiagen | 205311 |
| SYBR Green CFX for QPCR Applied Biosystems | Applied Biosystems | 4472942 |
| Seahorse XF Mitochondrial Stress Test | Agilent | 103015–100 |
| Proteasome Activity Fluorometric Assay Kit II | UBPBio | J4120 |
| CellROX green flow cytometry assay kit | Thermo Fisher | C10492 |
| Experimental Models: Cell Lines | ||
| HCT116 | ATCC | NCI-DTP Cat# HCT-116; RRID: CVCL_0291 |
| MCF7 | ATCC | NCI-DTP Cat# MCF7; RRID: CVCL_0031 |
| HT1080 | ATCC | CLS Cat# 300216/p517_HT-1080; RRID: CVCL_0317 |
| NCIH292 | Kindly provided from Dr. Joaquin Espinosa | ECACC Cat# 91091815; RRID: CVCL_0455 |
| MAF-794 | Kindly provided from Dr. Jean Mulcahy-Levy | N/A |
| BT549 | ATCC | NCI-DTP Cat# BT-549; RRID: CVCL_1092 |
| SJSA-1 | Kindly provided from Dr. Joaquin Espinosa | ATCC Cat# CRL-2098; RRID: CVCL_1697 |
| NCIH-1650 | ATCC | ATCC Cat# CRL-5883; RRID: CVCL_1483 |
| Experimental Models: Organisms/Strains | ||
| Athymic Nude mice (nu/nu) | Jackson Laboratory | Stock number: 007850 |
| Oligonucleotides | ||
| Oligonucleotides for qRT-PCR primers: Table S1 | this study; Fitzwalter et al., 2018; Oslowski and Urano, 2011; Radhakrishnan et al., 2010; Vangala et al., 2014. | N/A |
| Oligonucleotides for guide RNA design: Table S2 | this study | N/A |
| Recombinant DNA | ||
| LentiCRISPR – ATG7 | (O’Prey et al., 2017) | N/A |
| PLJM1-GFP-3xNLSpuromycin | (Thorburn et al., 2017) | N/A |
| PLJM1-mCherry-3xNLS blasticidin | (Thorburn et al., 2017) | N/A |
| PCDNA3-EV | this paper | N/A |
| PCDNA3-hATG7 isoform A | this paper | N/A |
| PLKO.1 shRNA-Keap1 TRCN0000158081 | Sigma-Aldrich | N/A |
| PLKO.1 shRNA-Keap1 TRCN0000158076 | Sigma-Aldrich | N/A |
| PLKO.1-shRNA-NRF2/NFE2L2 TRCN0000007559 | Sigma-Aldrich | N/A |
| PLKO.1-shRNA-NRF2/NFE2L2 TRCN0000007558 | Sigma-Aldrich | N/A |
| PLKO.1-shRNA-p62/SQSTM1 TRCN0000007234 | Sigma-Aldrich | N/A |
| PLKO.1-shRNA-p62 TRCN0000007236 | Sigma-Aldrich | N/A |
| NC16 pCDNA3.1 FLAG NRF2 | (Camp et al., 2012) | Addgene plasmid #36971 |
| Ub-G76V-GFP | (Dantuma et al., 2000) | Addgene plasmid #11941 |
| Other | ||
| Phusion High Fidelity DNA Polymerase | New England Biolabs | M0530L |
| CellEvent Caspase3/7 Green Detection Reagent | Thermo Fisher Scientific | C10423 |
| TransIT LT1 Transfection Reagent | Mirus | MIR 2304 |
| Cas9 recombinant protein | PNAbio | CP01 |