Figure 1.

Imaging Frema594 in whole-mount dura after i.v. injection of Frema594 or saline. (a) Schematic overview of the rat brain depicting areas of the overlying dura shown in Figures (b)–(d). (b) Saline injections induce no detectable fluorescent signal in the dura overlying the middle meningeal artery (MMA). (c) In the same area, distinct Frema594 labeling is seen in blood vessel walls (left) and axons (middle, right) 1 week after injection was made. (d) In the dura overlying the transverse sinus (TS), Frema594 labeling is seen throughout the dura at 4 hours and 1 week after injection (middle, right) but not after saline control (left). Green channel is shown for visualization of autofluorescence at 455/520 nm of unlabeled structures as an anatomical reference. Red channel shows Frema594 fluorescent signal at 551/624 nm. Scale bar = 100 µm.