Table 6. Nucleotide varition not differentiated by HRM.
| Primers | Haplotypes | Nucleotide difference | Specificity |
|---|---|---|---|
| MLT C1 - MLT C4 (TrnG intron) | C-D | T ↔ G & C ↔ A | 18 |
| B-C | A ↔ C | 20 | |
| B-D | T ↔ G | 29 | |
| A-C | GT ↔ TA, G ↔ T & T ↔ A | 88 | |
| A-D | G ↔ T & T ↔ A | 88 | |
| A-B | GT ↔ TA & G ↔ T | 93 | |
| MLT C3 - MLT C4 (TrnG intron) | A-D | T ↔ A | 11 |
| A-B | 8 bp indel & T ↔ A | 22 | |
| B-D | 8 bp indel | 33 | |
| B-C | T ↔ G & 8 bp indel | 65 | |
| C-D | G ↔ T | 73 | |
| A-C | T ↔ G & T ↔ A | 83 | |
| MLT M1 - MLT M2 (pctL-psbE intergenic spacer) | A-C | G ↔ T & A ↔ G | 6 |
| B-D | A ↔ G & G ↔ T | 93 | |
| MLT S1 - MLT S4 (atpI-atpH intergenic spacer) | A-C | 5 bp indel | 0 |
| MLT V1 - MLT V2 (rpl32-trnL intergenic spacer) | A-C | 6bp indel, T ↔ A & T ↔ A | 11 |
| A-D | 6 bp indel & T ↔ A | 93 | |
| C-D | A ↔ T | 96 |