Fig. 4. MiR-146a deficiency leads to increased proliferation of synovial fibroblasts.

A, In vitro proliferation of wt and miR-146a^−/− synovial fibroblasts, measured by ³[H] thymidine incorporation (data shown are representative of 3 independent experiments). B, Hematoxylin and eosin staining from histological sections of wt and miR-146a^−/− synovial fibroblasts cultured in micromasses (bars 500 μm, magnification x 5). C, Quantification of Ki-67⁺ synovial fibroblast cells among total synovial lining cells, from immunohistochemically stained sections of micromasses from wt (n = 7) and miR-146a^−/− (n = 7) mice using anti Ki-67 antibody. D, Expression level of TRAF6 in synovial fibroblasts from wt (n = 9) and miR-146a^−/− (n = 10) animals was analyzed using quantitative real time PCR. E and F, Synovial fibroblasts from wt or miR-146a deficient animals were transfected with either control (Ctrl) or TRAF6 siRNA, proliferation analysis was performed using ³[H]thymidine incorporation (data shown are mean values of 2 independent experiments). *p < 0,05, **p < 0,01, ***p < 0,001 Results are shown as mean ± SEM.