Table 1:
Antibody/ Probe |
Dilution | Fluorochrome | Distributor/Cat No. | Specificity |
---|---|---|---|---|
CD16/32 | 1:50 | BV-605 | BD Biosciences/563006 | Pan macrophage |
F4/80 | 1:400 | PE-Cy7 | eBioscience/25-4801 | Pan macrophage |
CD11b | 1:200 | Pacific Blue | BioLegend/101224 | Pan macrophage |
CD14 | 1:100 | PE | eBioscience/12-0141 | Pan macrophage |
Ly6G | 1:100 | APC | eBioscience/17-9668 | Neutrophils |
Siglec F | 1:64 | APC | Miltenyi Biotec/130-102-241 | Eosinophils |
CD19 | 1:400 | APC | eBioscience/17-0193 | B Lymphocytes |
CD3e | 1:800 | APC | eBioscience/17-0032 | T Lymphocytes |
Thy1.2 | 1:800 | APC | eBioscience/17/0902 | Fibroblasts |
CD31 | 1:200 | APC | BD Biosciences/551262 | Endothelial cells |
Ter119 | 1:200 | APC | eBioscience/17-5921 | Red blood cells |
PI | 1:200 | eBioscience 00-6990 |
Viability |