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Excitation and detection settings for antibodies and conjugates used. Exclusion markers were used for analysis of nerve samples.
| Antibody target | Conjugate | Excitation laser (nm) |
Emission Filters | Mirror |
|---|---|---|---|---|
| CD11b | Pacific Blue | 405 | 450/50 | |
| CD16/32 | BV605 | 405 | 585/42 | 570LP |
| eGFP | n/a | 488 | 515/20 | 505LP |
| CD14 | PE | 532 | 575/25 | |
| F4/80 | PE-Cy7 | 532 | 780/40 | 740LP |
| Propridium iodide | n/a | 532 | 660/20 | 640LP |
| Exclusion markers | APC | 633 | 660/20 |
