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. Author manuscript; available in PMC: 2020 May 18.
Published in final edited form as: Cell Rep. 2019 Jan 22;26(4):855–865.e5. doi: 10.1016/j.celrep.2019.01.003

KEY RESOURCES TABLE

REAGENT or RESOURCE SOURCE IDENTIFIER
Bacterial and Virus Strains
E. coli DH5α NEB C2989K
E. coli BL21-CodonPlus (DE3)-RIL Agilent Technologies 230245
Chemicals, Peptides, and Recombinant Proteins
BP clonase ThermoFisher 11789021
LR clonase ThermoFisher 11791043
Glutathione Sepharose FF GE Healthcare 17513201
HiLoad 16/600 Superdex 75 pg GE Healthcare 28–9893-33
HRV3 protease Sigma-Aldrich SΑE0045
KAR1 OlChemIm s.r.o. 025 7391
Chiralix CX27716
rac-GR24 OlChemIm s.r.o. 025 6721
rac-carlactone N/A
(+)-5-deoxystrigol OlChemIm s.r.o. 025 7121
(−)-5-deoxystrigol OlChemIm s.r.o. 025 7131
(+)-4-deoxyorobanchol OlChemIm s.r.o. 025 7141
(−)-4-deoxyorobanchol OlChemIm s.r.o. 025 7151
Sypro Orange 5,000X ThermoFisher S6650
Deposited Data
PpKAI2-like C This paper PDB: 6ATX
PpKAI2-like E This paper PDB: 6AZB
PpKAI2-like E S166A This paper PDB: 6AZC
PpKAI2-like H This paper PDB: 6AZD
Experimental Models: Organisms/Strains
KAI2:PpKAI2L-C/kai2 This paper N/A
KAI2:PpKAI2L-E/kai2 This paper N/A
KAI2:PpKAI2L-H/kai2 This paper N/A
KAI2:PpKAI2L-B/kai2 This paper N/A
KAI2:PpKAI2L-D/kai2 This paper N/A
KAI2:PpKAI2L-K/kai2 This paper N/A
KAI2:PpKAI2L-L/kai2 This paper N/A
KAI2:AtKAI2/kai2 This paper N/A
KAI2:AtKAI2SA/kai2 This paper N/A
35S:PpKAI2L-C/atd14 This paper N/A
35S:PpKAI2L-E/atd14 This paper N/A
35S:PpKAI2L-H/atd14 This paper N/A
35S:PpKAI2L-B/atd14 This paper N/A
Oligonucleotides
All oligonucleotides are listed in Table S2. N/A
Recombinant DNA
pGEX-GST-PpKAI2L-B This paper N/A
pGEX-GST-PpKAI2L-C This paper N/A
pGEX-GST-PpKAI2L-D This paper N/A
pGEX-GST-PpKAI2L-E This paper N/A
pGEX-GST-PpKAI2L-E S166A This paper N/A
pGEX-GST-PpKAI2L-E S166D S170D This paper N/A
pGEX-GST-PpKAI2L-E SDISDM166TDIHDE This paper N/A
pGEX-GST-PpKAI2L-E S97A This paper N/A
pGEX-GST-PpKAI2L-H This paper N/A
pGEX-GST-PpKAI2L-H TDIHDE167SDIDSM This paper N/A
pGEX-GST-PpKAI2L-H S98A This paper N/A
pGEX-GST-PpKAI2L-K This paper N/A
pGEX-GST-PpKAI2L-L This paper N/A
pGEX-GST-AtD14 This paper N/A
pGEX-GST-AtKAI2 This paper N/A
pENTR-PpKAI2L-C This paper N/A
pENTR-PpKAI2L-E This paper N/A
pENTR-PpKAI2L-H This paper N/A
pENTR-PpKAI2L-B This paper N/A
pENTR-PpKAI2L-D This paper N/A
pENTR-PpKAI2L-K This paper N/A
pENTR-PpKAI2L-L This paper N/A
pGWB1-KAI2Pro This paper N/A
pGWB1-KAI2Pro:PpKAI2L-C This paper N/A
pGWB1-KAI2Pro:PpKAI2L-E This paper N/A
pGWB1-KAI2Pro:PpKAI2L-H This paper N/A
pGWB1-KAI2Pro:PpKAI2L-B This paper N/A
pGWB1-KAI2Pro:PpKAI2L-D This paper N/A
pGWB1-KAI2Pro:PpKAI2L-K This paper N/A
pGWB1-KAI2Pro:PpKAI2L-L This paper N/A
pGWB2-PpKAI2L-C This paper N/A
pGWB2-PpKAI2L-E This paper N/A
pGWB2-PpKAI2L-H This paper N/A
pGWB2-PpKAI2L-B This paper N/A
Software and Algorithms
XDS Kabsch, 2010 http://xds.mpimf-heidelberg.mpg.de
Phenix Adams et al., 2010 https://www.phenix-online.org
Coot Emsley et al., 2010 https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot
CCP4mg McNicholas et al., 2011 http://www.ccp4.ac.uk/MG
ProteinsPlus Fährrolfes et al., 2017 https://proteins.plus
Origin Originlab https://www.originlab.com
XLfit IDBS https://www.idbs.com/excelcurvefitting
PRODRG Schuttelkopf and van Aalten, 2004 http://davapc1.bioch.dundee.ac.uk/cgi-bin/prodrg
AutoDock Vina Trott and Olson, 2010 http://vina.scripps.edu
Fiji Schindelin et al., 2012 https://fiji.sc
SPSS Statistics 22.0 IBM https://www.ibm.com/products/spss-statistics
RawConverter He et al., 2015 http://fields.scripps.edu/rawconv/
ProLuCID Xu et al., 2015 https://omictools.com/prolucid-tool
DTASelect Tabb et al., 2002 https://omictools.com/dtaselect-tool
Skyline MacLean et al., 2010 https://sciex.com/products/software/skyline-software