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. Author manuscript; available in PMC: 2021 Apr 21.
Published in final edited form as: Biochemistry. 2020 Apr 13;59(15):1537–1548. doi: 10.1021/acs.biochem.0c00107

Figure 7.

Figure 7.

Evaluation of the Leu-80 mutations of Adx on CYP24A1 function. (A) The L80K mutation of Adx disrupts EDC cross-linking with CYP11B1 and CYP11B2 enzymes, yet does not disrupt cross-linking with CYP24A1. (B) Fractional depletion of 1,25(OH)2D3 in reconstituted functional assays (triplicate samples) is represented by the box plot, indicating the presence of a functional contribution by the Leu-80 residue. (C) Nevertheless, L80Q and L80K result in comparable (L80Q) or higher (L80K) extent of NMR peak broadening for the resonance corresponding to Asp-76.