Fig 2. Sequence conservation/variation and evolutionary importance of HLA I peptide-binding groove positions.

(A) Sequence logo of the HLA I peptide-binding groove (residues 1–180). Polar, neutral, basic, acidic and hydrophobic amino acids are colored green, purple, blue, red, and black, respectively. (B) real-value Evolutionary Trace (rvET) scores of binding groove positions. Low rvET scores indicate high evolutionary importance, and vice versa.