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. Author manuscript; available in PMC: 2020 Jul 9.
Published in final edited form as: J Neuroendocrinol. 2019 Jul 9;31(7):e12763. doi: 10.1111/jne.12763

Figure 2. GHS-R deletion in AgRP neurons increases food anticipatory behavior and metabolic flexibility under calorie restriction.

Figure 2.

Indirect calorimetry analysis of mice under ad libitum feeding and after 12 weeks of 40% CR regimen. After a 7-day acclimatization period to metabolic cages, metabolic parameters were recorded for 3 days baseline, 24 hours fasting, and 24 hours refeeding. (A) Physical activity (beam breaks counts) for the 24 hours baseline recording before fasting. (B) Total physical activity (beam breaks per hour). (C) Physical activity in the 4 hours before onset of dark phase (expressed as beam breaks per hour); this is considered as food anticipatory activity (FAA). (D) Respiratory exchange ratio (RER, = VCO2/VO2 Ratio). (E) Average RER. For 40% CR group, mice from Cohort 1 were measured, n = 3–5 for each genotype. Two-way ANOVA (Diet and Genotype), with Šidák’s post hoc analysis. * p < 0.05.