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. 2020 May 12;11(19):1681–1690. doi: 10.18632/oncotarget.27564

Figure 4. CyTOF analysis of bone marrow cells following irradiation.

Figure 4

(A) Schematic representation of the treatment of mice prior to mass cytometry analysis (CyTOF) of the bone marrow compartment. Mouse thorax was irradiated, and the cells from femurs of mice were harvested day 1 and day 10 after irradiation, untreated mice were used as controls. (B) Representative density plots of lineage negative bone marrow cells in the tSNE1/tSNE2 fields following CyTOF acquisition. (C) Lineage-negative bone marrow cells in the tSNE1/tSNE2 fields displaying the median expression of CD11a. Color scale indicates the intensity of expression of CD11a. Minimum (min) and maximum (max) correspond to the 2nd and the 98th percentile values for each indicated marker, respectively. (D) Bar graph indicating the fold change in CD11a expression on Day 1 and Day 10 following thoracic IR. (E) Flow cytometry analysis of the bone marrow compartment following thoracic IR to validate the results of mass cytometry. The bar graph representing the counts of CD11a+ stem cells in the bone marrow. SD from at least three treatments. (F) Flow cytometry analysis of the bone marrow compartment following thoracic IR to evaluate for apoptosis. The bar graph representing the percentage of PARP positive cells in the bone marrow. SD from at least three treatments. (G) Irradiation mobilizes the cells expressing CD11a from the bone marrow into the circulation. The mouse thorax were irradiated (1.8 Gy ×5) and the cells expressing CD11a from the circulating blood were analyzed using flow cytometry at the time points indicated.