Table 5. Gene expression profiles in tumors from A-I KO are concordant with human melanoma gene signature for adverse outcome.
| Probe_ID | Gene Symbol | DEFINITION | A-IKO/ A-ITg (Mean) | p-value | Expression (in this study and in Oncomine’s) | Oncomine Datasets- Cutaneous Melanoma vs normal |
|---|---|---|---|---|---|---|
| ILMN_1231392 | Melk | Mus musculus maternal embryonic leucine zipper kinase (Melk), mRNA. | 1.5 | 0.002 | ↑↑ | Riker, Talantov |
| ILMN_2878355 | Kpna2 | Mus musculus karyopherin (importin) alpha 2 (Kpna2), mRNA. | 1.4 | 0.005 | ↑↑ | Riker, Talantov |
| ILMN_2714565 | Rrm2 | Mus musculus ribonucleotide reductase M2 polypeptide | 1.4 | 0.002 | ↑↑ | Riker, Talantov |
| ILMN_1214319 | Gemin6 | Mus musculus gem (nuclear organelle) associated protein 6 (Gemin6), mRNA. | 1.4 | 0.001 | ↑↑ | Riker, Talantov |
| ILMN_2632712 | Birc5 | Mus musculus baculoviral IAP repeat- containing 5 (Birc5), transcript variant 1, mRNA. | 1.4 | 0.006 | ↑↑ | Riker, Talantov |
| ILMN_2621422 | Kirrel | Mus musculus kin of IRRE like (Drosophila) (Kirrel), mRNA. | 1.3 | 0.026 | ↑↑ | Riker, Talantov |
| ILMN_2484707 | Tyms | Mus musculus thymidylate synthase (Tyms), mRNA. | 1.3 | 0.013 | ↑↑ | Riker, Talantov |
| ILMN_1218967 | Kif2c | Mus musculus kinesin family member 2C (Kif2c), mRNA. XM_986361 | 1.3 | 0.015 | ↑↑ | Riker, Talantov |
| ILMN_1233857 | Mcm6 | Mus musculus minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe) (S. cerevisiae) (Mcm6), mRNA. | 1.3 | 0.012 | ↑↑ | Riker, Talantov |
| ILMN_2797642 | Ncaph/ Brrn1 | Mus musculus non-SMC condensin I complex, subunit H (Ncaph), mRNA. | 1.3 | 0.013 | ↑↑ | Riker, Talantov |
| ILMN_2677595 | Ncapg2/Mtb | Mus musculus non-SMC condensin II complex, subunit G2 (Ncapg2), mRNA. | 1.2 | 0.022 | ↑↑ | Riker, Talantov |
| ILMN_1245757 | Nans | Mus musculus N-acetylneuraminic acid synthase (sialic acid synthase) (Nans), mRNA. | 1.2 | 0.027 | ↑↑ | Riker, Talantov |
| ILMN_2804444 | Pcdh17 | Mus musculus protocadherin 17 (Pcdh17), mRNA. | 1.5 | 0.001 | ↑↑ | Riker |
| ILMN_2688944 | Htra2/ Prss25 | Mus musculus HtrA serine peptidase 2 (Htra2), nuclear gene encoding mitochondrial protein, mRNA. | 1.4 | 0.001 | ↑↑ | Riker |
| ILMN_2741050 | Rfc4 | Mus musculus replication factor C (activator 1) 4 (Rfc4), mRNA. | 1.4 | 0.013 | ↑↑ | Riker |
| ILMN_1257552 | Tars | Mus musculus threonyl-tRNA synthetase (Tars), mRNA. | 1.4 | 0.008 | ↑↑ | Riker |
| ILMN_3097131 | Timeless | Mus musculus timeless homolog (Drosophila) (Timeless), transcript variant 2, mRNA. | 1.3 | 0.013 | ↑↑ | Riker |
| ILMN_2975640 | Snrpg | Mus musculus small nuclear ribonucleoprotein polypeptide G (Snrpg), mRNA. | 1.2 | 0.013 | ↑↑ | Riker |
| ILMN_2711112 | Shcbp1 | Mus musculus Shc SH2-domain binding protein 1 (Shcbp1), mRNA. | 1.4 | 0.001 | ↑↑ | Talantov |
| ILMN_2607926 | Kdelr2 | Mus musculus KDEL (Lys-Asp-Glu-Leu) endoplasmic reticulum protein retention receptor 2 (Kdelr2), mRNA. | 1.4 | 0.002 | ↑↑ | Talantov |
| ILMN_2816754 | Ndc80/ Kntc2 | Mus musculus NDC80 homolog, kinetochore complex component (S. cerevisiae) (Ndc80), mRNA. | 1.4 | 0.006 | ↑↑ | Talantov |
| ILMN_2971845 | Dtymk | Mus musculus deoxythymidylate kinase (Dtymk), mRNA. | 1.4 | 0.006 | ↑↑ | Talantov |
| ILMN_2588362 | Cdca8 | Mus musculus cell division cycle associated 8 (Cdca8), mRNA. | 1.4 | 0.003 | ↑↑ | Talantov |
| ILMN_2934457 | Ran | Mus musculus RAN, member RAS oncogene family (Ran), mRNA. | 1.4 | 0.004 | ↑↑ | Talantov |
| ILMN_2999654 | Psmc3ip | Mus musculus proteasome (prosome, macropain) 26S subunit, ATPase 3, interacting protein (Psmc3ip), mRNA. | 1.4 | 0.003 | ↑↑ | Talantov |
| ILMN_2598852 | Ranbp1 | Mus musculus RAN binding protein 1 (Ranbp1), mRNA. | 1.3 | 0.007 | ↑↑ | Talantov |
| ILMN_2639036 | Hspd1 | Mus musculus heat shock protein 1 (chaperonin) (Hspd1), mRNA. | 1.3 | 0.011 | ↑↑ | Talantov |
| ILMN_2745005 | Gpn3/ MGC14 560 | Mus musculus GPN-loop GTPase 3 (Gpn3), mRNA. | 1.3 | 0.005 | ↑↑ | Talantov |
| ILMN_2919433 | Cdc45l/Cdc45 | Mus musculus cell division cycle 45 homolog (S. cerevisiae)-like (Cdc45l), mRNA. | 1.3 | 0.043 | ↑↑ | Talantov |
| ILMN_2749937 | Ncoa6 | Mus musculus nuclear receptor coactivator 6 (Ncoa6), mRNA. | 1.3 | 0.011 | ↑↓ | Talantov |
| ILMN_2605890 | Tk1 | Mus musculus thymidine kinase 1 (Tk1), mRNA. | 1.3 | 0.036 | ↑↑ | Talantov |
| ILMN_2706882 | Donson | Mus musculus downstream neighbor of SON (Donson), mRNA. | 1.3 | 0.028 | ↑↑ | Talantov |
| ILMN_2680648 | Atad2 | Mus musculus ATPase family, AAA domain containing 2 (Atad2), mRNA. | 1.3 | 0.026 | ↑↑ | Talantov |
| ILMN_2657844 | Cdc2a/ Cdk1 | Mus musculus cell division cycle 2 homolog A (S. pombe) (Cdc2a), mRNA. | 1.3 | 0.021 | ↑↑ | Talantov |
| ILMN_2608933 | Tcof1 | Mus musculus Treacher Collins Franceschetti syndrome 1, homolog (Tcof1), mRNA. | 1.2 | 0.030 | ↑↑ | Talantov |
| ILMN_1233065 | Rbmx | Mus musculus RNA binding motif protein, X chromosome (Rbmx), mRNA. | 1.2 | 0.053 | ↑↑ | Talantov |
| ILMN_1236574 | Cenpa | Mus musculus centromere protein A (Cenpa), mRNA. | 1.2 | 0.016 | ↑↑ | Talantov |
| ILMN_1221067 | Nme1 | Mus musculus non-metastatic cells 1, protein (NM23A) expressed in (Nme1), mRNA. | 1.2 | 0.006 | ↑↑ | Talantov |
| ILMN_2830661 | Top2a | Mus musculus topoisomerase (DNA) II alpha (Top2a), mRNA. | 1.2 | 0.025 | ↑↑ | Talantov |
| ILMN_2936427 | Mcm4 | Mus musculus minichromosome maintenance deficient 4 homolog (S. cerevisiae) (Mcm4), mRNA. | 1.2 | 0.050 | ↑↑ | Talantov |
| ILMN_2688912 | F10 | Mus musculus coagulation factor X | 0.4 | 0.012 | ↓↓ | Talantov |
Forty-one (41) out of 58 genes (from the comparison analysis of our experiment [ten A-I KO vs ten A-I Tg+/– tumors] with human biopsies studies that associated with adverse prognosis) were observed to be differentially regulated (> 2-fold) and statistically significant (p ≤ 0.01) in Cutaneous Melanoma vs Normal samples (from either both or one of the two datasets -Riker and Talantov) in Oncomine database. The Riker (Human Genome U133 Plus 2.0 Array, mRNA [40];) has 14 melanoma and 4 normal samples; Talantov (Human Genome U133A Array, mRNA [7];) has 45 melanoma and 7 normal samples.