Table 1.

Spearman’s rank correlation coefficients of eupyrene and apyrene sperm ejaculated in relation to copulation duration

		Copulation duration
Sperm	Treatment	1st mating	2nd mating	3rd mating	4th mating
Eupyrene	CONT	0.2948 ns	−0.1978 ns	0.0250 ns	0.0140 ns
	+M	−0.2901 ns	0.4707 ns	0.2590 ns	−0.0364 ns
	+F	0.0546 ns	−0.1436 ns	−0.1101 ns	0.5252 *
Apyrene	CONT	0.0018 ns	−0.0022 ns	−0.1271 ns	−0.2281 ns
	+M	0.1360 ns	0.2645 ns	0.0751 ns	−0.3242 ns
	+F	−0.2359 ns	−0.0786 ns	0.1360 ns	0.5058 ns

^nsnot significantly different (P > 0.05) and

^*significantly different (P < 0.05): eupyrene or apyrene sperm number versus copulation duration among treatments in each of the first four copulations.