Table 4.
Tissue concentrationsa (pM) of [125I]-IgG following intranasal saline (control) vs MMP-9 (100 nM) pre-treatment.
| Intranasal (saline pre-treatment) | Intranasal (MMP-9 pre-treatment) | |
|---|---|---|
| Blood | (pM) | (pM) |
| 0 min | 0 (4) | 0 (5) |
| 10 min | 10.63 ± 0.79 (4) | 18.42 ± 3.29 (5) |
| 20 min | 26.86 ± 1.48 (4) | 34.72 ± 6.31 (5) |
| 30 min | 37.94 ± 2.71 (4) | 50.64 ± 9.75 (5) |
| Blood AUC (0–30 min) | (pM*min) | (pM*min) |
| 562.27 ± 38.68 (4) | 784.65 ± 140.63 (5) | |
| Nervous system regions | (pM) | (pM) |
| ACA & MCA b | 18.55 ± 4.44 (4) d | 71.49 ± 21.00 (4) |
| Basilar & vertebral arteries c | 26.61 ± 14.00 (5) | 83.78 ± 54.36 (6) |
| Olfactory bulbs | 4.66 ± 1.59 (5) e | 23.86 ± 6.89 (4) |
| Frontal cortex | 2.36 ± 0.75 (5) f | 21.71 ± 12.62 (4) |
| Caudoputamen | 1.01 ± 0.17 (4) d | 7.51 ± 2.38(4) |
| Motor cortex | 1.39 ± 0.34 (4) g | 8.28 ± 2.00 (6) |
| Primary somatosensory cortex | 2.13 ± 0.49 (5) h | 5.73 ± 1.42 (4) |
| Midbrain | 1.38 ± 0.32 (5) | 4.31 ± 1.68 (3) |
| Posterior hippocampus | 2.45 ± 0.88 (5) | 8.91 ± 1.29 (4) |
| Medulla | 1.24 ± 0.21 (5) | 2.62 ± 0.88 (4) |
| Pons | 1.82 ± 0.52 (5) i | 5.85 ± 1.61 (3) |
| Cerebellum | 1.98 ± 0.53 (5) | 3.30 ± 0.58 (4) |
| Cervical spinal cord | 1.73 ± 0.16 (4) d | 14.82 ± 6.74 (4) |
| Trigeminal nerves | 111.25 ± 53.21 (5) | 154.66 ± 33.69 (6) |
| Lymph nodes | (pM) | (pM) |
| Deep cervical lymph nodes | 151.19 ± 78.77 (5) | 304.33 ± 228.74 (4) |
| Superficial cervical lymph nodes | 81.24 ± 43.13 (5) f | 15.05 ± 4.92 (4) |
| Axillary lymph nodes | 4.82 ± 1.19 (5) | 12.59 ± 1.08 (2) |
Blood, nervous system, and lymph node radiolabel concentrations following intranasal administration of [125I]-IgG following saline (control) or MMP-9 (100 nM) pre-treatment. Intranasal administration of saline or MMP-9 solution was first performed drop wise (4 drops of 6 μl each) over approximately 15 min, followed by intranasal administration of a low tracer dose (50 μg) of IgG - performed drop wise (4 drops of 6 μl each) over approximately 15 min.
Data are presented as mean ± S.E.M. (n independent experiments).
anterior cerebral and middle cerebral arteries
basilar and vertebral arteries. Statistical analysis for tissue samples - saline vs MMP-9 pre-treatment: Mann-Whitney Rank sum test –
Mann-Whitney U = 0, p = .029;
t (7) = 3.039, p = .019;
Mann-Whitney U = 0, p = .016;
Mann-Whitney U = 0, p = .010; Two-tailed Studenťs t-test –
t(7) = 2.636, p = .034;
t(6) = 2.955, p = .025.