Figure 1. HES contains a factor, distinct from HpARI, which suppresses detection of ST2.

(A–B) HpARI (5 μg) or HES (10 μg) were coadministered with 25 μg of Alternaria allergen by the intranasal route, and lung cell ST2 staining assessed 24 hr later. Geometric mean fluorescence intensity (MFI) of ST2 staining on ILC2 (ICOS⁺lineage^–CD45⁺) is shown in (A), with representative histograms shown in (B). Representative of 2 replicate experiments, each with 3–5 mice per group. Error bars show SEM. (C) Naive murine lung cells were cultured for 24 hr in the presence of HES at the concentrations indicated, after which ST2 MFI on ILC2 was assessed. Data representative of >3 repeat experiments, n = 3 per group.