Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background, Mus musculus | ST2-deficient; Il1rl1-/- | (Mangan et al., 2007) | BALB/c background | |
| Cell line (Homo sapiens) | Expi293F | ThermoFisher Scientific | Cat No: A14527 | |
| Recombinant DNA reagent | pSecTAG2A vector | ThermoFisher Scientific | Cat No: V90020 | |
| Other | Alternaria alternata allergen extract | Greer | Cat No: XPM1D3A25 | |
| Peptide, recombinant protein | Mouse IL-2 | Biolegend | Cat No: 575402 | |
| Peptide, recombinant protein | Mouse IL-7 | Biolegend | Cat No: 577802 | |
| Peptide, recombinant protein | Mouse IL-33 | Biolegend | Cat No: 580502 | |
| Peptide, recombinant protein | Mouse IL-25 | Biolegend | Cat No: 587302 | |
| Peptide, recombinant protein | Human IL-33 | Biolegend | Cat No: 581802 | |
| Peptide, recombinant protein | Mouse ST2-Fc chimera | Biolegend | Cat No: 764902 | |
| Peptide, recombinant protein | Human ST2-Fc chimera | Biolegend | Cat No: 557904 | |
| Antibody | Anti-CD3-FITC (Armenian hamster monoclonal) | Biolegend | Cat No: 100306 RRID:AB_312671 Clone: 145–2 C11 |
(1:200) |
| Antibody | Anti-CD5-FITC (Rat monoclonal) | Biolegend | Cat No: 100606 RRID:AB_312735 Clone: 53–7.3 |
(1:200) |
| Antibody | Anti-CD11b-FITC (Rat monoclonal) | Biolegend | Cat No: 101224 RRID:AB_755986 Clone: M1/70 |
(1:200) |
| Antibody | Anti-CD19-FITC (Rat monoclonal) | Biolegend | Cat No: 115506 RRID:AB_313641 Clone: 6D5 |
(1:200) |
| Antibody | Anti-CD49b-FITC (Rat monoclonal) | ThermoFisher Scientific | Cat No: 11-5971-85 RRID:AB_465327 Clone: DX5 |
(1:200) |
| Antibody | Anti-GR1-FITC (Rat monoclonal) | Biolegend | Cat No: 108406 RRID:AB_313371 Clone: RB6-8C5 |
(1:200) |
| Antibody | Anti-CD45-AlexaFluor700 (Rat monoclonal) | Biolegend | Cat No: 103128 RRID:AB_493715 Clone: 30-F11 |
(1:200) |
| Antibody | Anti-CD45-Pacific Blue (Rat monoclonal) | Biolegend | Cat No: 103126 RRID:AB_493535 Clone: 30-F11 |
(1:200) |
| Antibody | Anti-ICOS-PCP-Cy5.5 (Armenian hamster monoclonal) | Biolegend | Cat No: 313518 RRID:AB_10641280 Clone: C398.4A |
(1:100) |
| Antibody | Anti-CD4-PE-Dazzle (Rat monoclonal) | Biolegend | Cat No: 100566 RRID:AB_2563685 Clone: RM4-5 |
|
| Antibody | Anti-ST2-APC (Rat monoclonal) | ThermoFisher Scientific | Cat No: 17-9335-82 RRID:AB_2573301 Clone: RMST2-2 |
(1:100) Used throughout manuscript. |
| Antibody | Anti-ST2-APC (Rat monoclonal) | Biolegend | Cat No: 145306 RRID:AB_2561917 Clone: DIH9 |
(1:100) Used in Figure 7—figure supplement 1 only |
| Antibody | Anti-CD11c-AlexaFluor647 (Armenian hamster monoclonal) | Biolegend | Cat No: 117312 RRID:AB_389328 Clone: N418 |
(1:200) |
| Antibody | Anti-SiglecF-PE (Rat monoclonal) | Miltenyi | Cat No: 130-102-274 RRID:AB_2653451 Clone: ES22-10D8 |
(1:50) |
| Antibody | Anti-KLRG1-PCP-Cy5.5 (Syrian hamster monoclonal | Biolegend | Cat No: 138417 RRID:AB_2563015 Clone: 2F1 |
(1:100) |
| Antibody | Anti-IL-5-PE (Rat monoclonal) | Biolegend | Cat No: 504304 RRID:AB_315328 Clone: TRFK5 |
(1:200) |
| Antibody | Anti-IL-13-PE-Cy7 (Rat monoclonal) | ThermoFisher Scientific | Cat No: 25-7133-82 RRID:AB_2573530 Clone eBio13A |
(1:200) |
| Peptide, recombinant protein | Streptavidin-PE | Biolegend | Cat No: 405203 | |
| Kit | Live/dead fixable blue | ThermoFisher Scientific | Cat No: L34962 |