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. 2020 Apr 21;9:e53244. doi: 10.7554/eLife.53244

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© 2020, Murata et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Author response image 2. — Peripheral T cells from HLA-matched healthy donors were stained with pHLA multimers that positively stained TILs for HLA-A*02:01⁺ (A), A*24:02⁺ (B), B*07:02⁺ (C), B*18:01⁺ (D), B*40:02⁺ (E), B*41:01⁺ (E), C*03:04⁺ (F), C*05:01⁺ (G), C*06:02⁺ (H), and C*07:01⁺ (I) healthy donors. The A*02:01/Flu MP_58-66 (A), A*24:02/EBV LMP2_222-230 (B), and B*07:02/EBV EBNA3A_379-387 (C) multimers were employed as positive controls. The A*02:01/HTLV-1 tax_11-19 (A), A*24:02/HTLV-1 tax_301-309 (B), B*07:02/HIV nef_128-137 (C), B*18:01/HIV gag_161-170 (D), B*40:01/HIV nef_92-100 (E), C*03:04/HIV gag_164-172 (F), C*05:01/HIV rev_67-75 (G), C*06:02/HIV nef_120-128 (H), and C*07:01/HIV nef_105-115 (I) multimers were used as negative controls. The percentage of multimer⁺ cells in CD8⁺ T cells is shown.