Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (Homo-sapiens) | K562 | ATCC | RRID:CVCL_0004 | |
| Cell line (Homo-sapiens) | T2 | ATCC | RRID:CVCL_2211 | |
| Cell line (Homo-sapiens) | A375 | ATCC | RRID:CVCL_0132 | |
| Cell line (Homo-sapiens) | Malme-3M | ATCC | RRID:CVCL_1438 | |
| Cell line (Homo-sapiens) | SK-MEL-28 | ATCC | RRID:CVCL_0526 | |
| Cell line (Homo-sapiens) | LM-MEL-53 | ATCC | RRID:CVCL_UC56 | |
| Cell line (Homo-sapiens) | HEK293T | ATCC | RRIID:CVCL_0063 | |
| Cell line (Homo-sapiens) | MCF7 | ATCC | RRID:CVCL_UC56 | |
| Cell line (Homo-sapiens) | ACHN | ATCC | RRID:CVCL_1067 | |
| Cell line (Homo-sapiens) | SK-MEL-21 | Memorial Sloan Kettering Cancer Center | RRID:CVCL_3877 | |
| Cell line (Homo-sapiens) | SK-MEL-37 | Memorial Sloan Kettering Cancer Center | RRID:CVCL_3878 | |
| Cell line (Homo-sapiens) | Me275 | Ludwig Institute for Cancer Research | RRID:CVCL_S597 | |
| Cell line (Homo-sapiens) | Jurkat 76 | Leiden University Medical Center | Heemskerk et al. (2003) | |
| Antibody | Mouse anti-CD8-PC5 | Beckman Coulter | Cat.#: IM2638U | Clone B9.11 |
| Antibody | Mouse CD8-PB | Beckman Coulter | Cat.#: A82791 | Clone B9.11 |
| Antibody | Mouse anti-NGFR-FITC | Biolegend | Cat.#: MAB5598 | Clone ME20.4 |
| Antibody | Mouse anti-CD3-APC/Cy7 | Biolegend | Cat.#: 300426 | Clone UCHT1 |
| Antibody | Mouse anti-HLA-A2-FITC | Biolegend | Cat.#: 343304 | Clone BB7.2 |
| Antibody | Mouse anti-Melan-A | Santa Cruz Biotechnology |
Cat.#: sc-20032 | Clone A103 |
| Antibody | Rabbit anti-NY-ESO-1-PE | Cell Signaling Technology | Cat.#: 65699 | Clone D1Q2U |
| Antibody | Mouse anti-PMEL | LifeSpan Biosciences | Cat.#: LS-C114528 | Clone 7E3 |
| Antibody | Mouse anti-MAGE-A1 | LifeSpan Biosciences | Cat.#: LS-C391567 | Clone MA454 |
| Antibody | Rabbit anti-Tyrosinase | Abcam | Cat.#: ab61294 | Clone ERP10141 |
| Antibody | Rabbit anti-SSX2 | Thermo Fisher Scientific | Cat.#: PA5-80074 | Polyclonal |
| Antibody | Rabbit anti-MAGE-A3 | LifeSpan Biosciences | Cat.#: LS-C99934 | Polyclonal |
| Antibody | Mouse anti-β-actin | Santa Cruz Biotechnology | Cat.#: sc-47778 | Clone C4 |
| Antibody | Mouse anti-His tag-PE | Abcam | Cat.#: ab72467 | Clone AD1.1.10 |
| Antibody | Mouse anti-His tag-APC | Abcam | Cat.#: ab72579 |
Clone AD1.1.10 |
| Commercial assay or kit |
LIVE/DEAD Fixable Aqua Dead Cell Stain kit | Thermo Fisher Scientific |
at.#: L34957 | |
| Commercial assay or kit | PureLink Genomic DNA Mini Kit | Thermo Fisher Scientific | Cat.#: K182000 | |
| Commercial assay or kit | Cytofix/Cytoperm kit | BD Biosciences | Cat.#: BD 554714 | |
| Commercial assay or kit | CD8+ T Cell Isolation Kit | Miltenyi Biotec | Cat.#: 130-096-495 | |
| Commercial assay or kit | Pan T Cell Isolation Kit | Miltenyi Biotec | Cat.#: 130-096-535 | |
| Software, algorithm | Cancer Antigenic Peptide Database | Cancer Research Institute | https://caped.icp.ucl.ac.be | |
| Software, algorithm | NetMHC 4.0 | Andreatta and Nielsen (2016) | http://www.cbs.dtu.dk/services/NetMHC/ |