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. Author manuscript; available in PMC: 2020 May 18.
Published in final edited form as: Biochem J. 2015 May 15;468(1):49–63. doi: 10.1042/BJ20140697

Figure 2. Levels of TCA-cycle intermediates are less glucose responsive in αTC1-6 than in INS-1 832/13.

Figure 2

(A) A score-scatter plot for an OPLS-DA calculated with the cell-type (αTC1-6 or INS-1 832/13) and the stimulatory glucose level as discriminating variables. Each point in this plot corresponds to a sample; the position of the point is determined by levels of all detected metabolites. (B) The loading-scatter plot corresponding to the score-scatter plot shown in (A) reveals which changes in metabolite levels that underlie the clustering of samples observed in (A). (C) Fold-changes in metabolite levels after stimulation of αTC1-6 and INS-1 832/13-cells with 16.7 mM glucose. Data are shown for αTC1-6 (n=12) and INS-1 832/13 (n=11) in (A-C), expressed as the average fold-change to basal glucose levels ± SEM in (C). Statistical significance was assessed using ANOVA and the paired (within cell-type) and unpaired (between cell-types) Student's t-test. 2.8 vs 16.7 mM glucose for INS-1 832/13, *p<0.05, **p<0.01, ***p<0.001. 1 vs 16.7 mM glucose for αTC1-6, #p<0.05, ##p<0.01, ###p<0.001. Fold-change for αTC1-6 vs INS-1 832/13, $$p<0.01, $$$p<0.001. First (t[1]) and second (t[2]) predictive component; first (p[1]) and second (p[2]) predictive loadings scaled as correlations. Glycolytic and TCA-cycle intermediates are indicated by ellipses. Metabolites discussed in the text are shown in black, additional metabolites are shown in grey. Mal, malate; Fum, fumarate; Cit, citrate; IsoCit, isocitrate; Succ, succinate; AKGA, α-ketoglutarate; Ala, alanine; Pyr, pyruvate; Lac, lactate; Gluc6P, glucose-6-phosphate; Fruct6P, fructose-6-phosphate; GlyA3P, 3-phosphoglycerate; Gly3P, glycerol-3-phosphate; Asp, aspartate; Glu, glutamate; Rib5P, ribose-5-phosphate; Pglu, pyruglutamate; Ser, serine; Lys, lysine; Thr, threonine; Gly2P, glycerol-2-phosphate; Amal, aminomalonate; Meth, methionine; Val, valine; Tau, taurine; PP, pyrophosphate; Ile, isoleucine; Cys, cysteine; Chol, cholesterol; Ura, uracil; Bala, beta-alanine; Pro, proline; SPAll, all detected hexose phosphates; Mino, myo inositol; Fruct, fructose; InosP, inositolphosphate; OctOl, octadecanol; GlycolA, glycolate; OxalA, oxalate; GABA, gamma-aminobutyrate; Phe, phenylalanine; Gly, glycine; Creat, creatinine; BenzA, benzoate; AdipA, adipate; C10:0, caproate; C12:0, laurate; C14:0, myristate; C16:0, palmitate; C17:0, heptadecanoate; C18:0, stearate; C18:1, oleate; C20:0, arachidate; C20:4, arachidonate.