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. 2020 Mar 16;45(7):1159–1170. doi: 10.1038/s41386-020-0656-5

Fig. 4. Expression of excitatory DREADD in PNs enhances their firing activity in the BTBR cerebellar cortex.

Fig. 4

a Example of AAV-carried DREADD expression in the BTBR cerebellum. b Selective targeting of PNs by AAV8-Pcp2-hM3Dq-mCherry (hM3Dq). ML molecular layer, GL granular layer. c APs elicited from PNs transduced with hM3Dq (left) or AAV8-Pcp2-mCherry (mCherry, right) before (blue) and after bath application of CNO (10 µM, magenta). d Frequencies of APs are quantified for the above conditions (n = 9 for hM3Dq, n = 6 for mCherry). e APs generated by depolarization steps (top) from a PN transduced with hM3Dq (left) or mCherry (right) before (blue) and after CNO perfusion (10 µM, magenta) in cocktail blockers of NBQX (10 μM), APV (50 μM) and bicuculline (10 μM). f, g Changes in membrane potentials (left) and numbers of spikes (right) made by CNO are summarized for hM3Dq (f, n = 10) and mCherry (g, n = 10) groups. Solid lines represent fits to a Boltzmann function: f(I) = Vmax/(1 + e(Imid-I)/Ic)+C, in which “Vmax” is theoretical value of the maximal number of APs, “Imid” is depolarization current needed to produce half of the maximal number of APs, and “Ic” is steepness of the Boltzmann curve.