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. 2020 May 18;10:8182. doi: 10.1038/s41598-020-65182-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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UGCG overexpression impact on mitochondrial dynamics and mitochondrial and cytoplasmic enzymes. (A) The mRNA expression of the pro-fission enzymes mitofusin 1 (MFN1) and mitofusin 2 (MFN2) was determined by qRT-PCR analysis. The expression is related to the housekeeping gene (RPL37A). Data are presented as a mean of n = 3 ± SEM. (B) Protein concentration of MFN1, MFN2 and optic atrophy type 1 (OPA1) determined by Western blot analysis. Data are related to the housekeeping protein HSP90 and presented relative to MCF-7/empty control cells as a mean of n = 6 ± SEM. (C) mRNA levels of the pro-fission enzymes PTEN-induced kinase 1 (PINK1) and mitochondrial fission protein 1 (FIS1) related to RPL37A. Data are presented as a mean of n = 3 ± SEM. (D) Protein concentration determination of total dynamin related protein1 (DRP1), total mitochondrial fission factor (MFF) and phospho-DRP1 (Ser616) by Western blot analysis. Data are related to the housekeeping protein HSP90 and presented relative to MCF-7/empty control cells as a mean of n = 6 ± SEM. (E) mRNA expression analysis of alpha-ketoglutarate dehydrogenase (OGDH), pyruvate dehydrogenase E1 component subunit alpha (PDHA1), and beta (PDHB) and glutamate oxaloacetate transaminase 2 (GOT2) by qRT-PCR. The expression is related to RPL37A. Data are presented as a mean of n = 3 ± SEM. (F) mRNA expression analysis of glutamate oxaloacetate transaminase 1 (GOT1) and lactate dehydrogenase subunit A (LDHA) by qRT-PCR. The expression is related to RPL37A. Data are presented as a mean of n = 3 ± SEM. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.