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. 2020 May 18;11:2460. doi: 10.1038/s41467-020-16297-3

Fig. 3. Quantification and visualization of the fluorescence lifetime in photo-switchable nanogels.

Fig. 3

a Fluorescence decay of nanogels (N1, blue, N2, red, N3, pink, N4, yellow) after UV irradiation for 120 s (λex. = 405 nm); Bottom: corresponding residuals. The sum of the three-exponential fitting with calculated averaged lifetime evaluation is given in Supplementary Information (Supplementary Table 8); b Fluorescence lifetime histogram of the nanogel solutions (N1-N4) after UV irradiation (λex = 410 nm); c The average lifetime (τAv,I) of nanogel solution (N4) after UV irradiating for 0–50 s; d Reversible 2D fluorescence decay of N4 upon alternating the UV and visible irradiation for six times (λex. = 375 nm, λem. = 520 nm); e Fluorescence lifetime mapping of nanogel solutions (N1-4, 1 mg mL−1) before and after UV irradiation for 120 s (λex. = 405 nm, λem. = 520 nm). Right: FLIM map showing the reversible lifetime of N4 in solution through alternating the UV and visible irradiation (λex. = 405 nm, irradiation time: 120 s). Color bar: Fast lifetimes (ns); Similar images were obtained for more than two sets of experiments.