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. 2020 Jan 20;45(7):1115–1124. doi: 10.1038/s41386-020-0616-0

Fig. 3. Inhibition of DRN-projecting LHb neurons decreases perseverative saccharin seeking.

Fig. 3

a Experimental timeline and legend. b, f, j, n All animals learned to increase saccharin licking across four training sessions in a two-bottle choice lickometer chamber. d, h, l Inhibition of the LHb output pathways (DRN, n = 8 paired; VTA, n = 11 paired; RMTg, n = 11 paired) had no effect on saccharin licking, p and CNO also had no effect on saccharin licking in animals lacking DREADD receptors (n = 9, paired). d During a reward-omission test, inhibition of the LHb to DRN pathway reduced licking at a previously saccharin-paired bottle (CNO n = 4, Veh n = 4; d = 2.45; F(5,1) = −16.00, p = 0.007). e During a reward-omission test, inhibition of the LHb to DRN pathway also increased licking at a previously water-paired bottle (CNO n = 4, Veh n = 4; d = 2.02; F(5,1) = −10.90, p = 0.016). h, i, l, m Inhibition of LHb outputs to VTA (CNO n = 7, Veh n = 4; outlier not shown) and RMTg (CNO n = 6, Veh n = 5) had no effect on the reward-omission test. p, q CNO also had no effect on reward-omission testing in animals lacking DREADD expression (CNO n = 5, Veh n = 4). *p < 0.05; **p < 0.01.