Fig. 2. α1D-AR pathway is not involved in Naftopidil-induced BH3-only proteins.
a Expression of α1, α1A, α1B and α1D-AR proteins was assessed by western blot for SKOV3 and IGROV1-R10 cell lines. The effect of increasing concentrations of BMY-7378 (0, 10, 100 µM) on SKOV3 (left column) and IGROV1-R10 (right column) cells was analyzed (b) after a 24- and 48-h treatment on proliferation by the Trypan blue exclusion test (curves represent the relative viable cells number in the treated cells normalized to that in T0 condition) (left and right panels) and after a 48-h treatment on the BH3-only members expression by western blot (middle panel). The impact of the Methoxamine (100 µM) and Naftopidil (50 µM) combination was analyzed (c) after a 24- and 48-h treatment on proliferation by the Trypan blue exclusion test (curves represent the relative viable cells number in the treated cells normalized to that in T0 condition) (left and right panels) and after a 48-h treatment on the BH3-only member expression by western blot (middle panel) (N = 3). Results were considered statistically different if *p < 0.05, **p < 0.01, ***p < 0.001.