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. 2020 May 18;10:8145. doi: 10.1038/s41598-020-65042-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Validation results of circulating lymphocytes and monocytes by RT-qPCR in DL-P versus H (A), P versus H (B), T2DMpoorly-DL-P versus H (C), DL-P versus H (D) and P versus H (E). All mRNA levels of the investigated genes were normalized to a mean of the endogenous controls GAPDH, ACTG1 and RPL7A genes. Data represent the mean ± SD of 30 patients per group (Mann-Whitney U test; α = 5%). *significant p values compared to H. (A) CD47 gene, p values <0.0001; DAB2 gene, p values <0.0001. (B) ITGB2 gene, p values <0.0001; IGHDL-P gene, p values = 0.0053. (C) HLADRB4 gene, p values <0.0001. (D) HLADRB4 gene, p values = 0.0018. (E) HLADRB4 gene, p values = 0.0002.