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. 2020 May 18;10:8178. doi: 10.1038/s41598-020-65180-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Maternal LB exposure promotes neuronal and oligodendrocyte precursor cell development in the offspring. Proteins of two weeks old cortex tissues were separated on SDS-PAGE gels and probed with NeuN, NFL and Syn1 for neuronal development (a) and NG2, Olig2, MBP for oligodendrocyte development (b). For both (a) and (b), top: representative raw images of western blot analysis; bottom: scatter plots of densitometry results normalized to GAPDH displayed as a fold of control. Unpaired t test with Welch’s correction was used to detect the difference between SPF (n = 7) and LB (n-6) group. NeuN-Neuronal Nuclei; NFL-Neurofilament-L; Syn1-Synapsin; NG2-Neuroglia antigen 2; Olig2-Oligodendrocyte Transcription Factor 2; MBP-Myelin Basic Protein. Bars with ⎴ denote significant difference between experimental groups (at least p < 0.05). Blots were cropped and the original blots are presented in Supplementary Figure S3.