Table 1.
Platelet concentration (thou/μL) |
Platelet fold change |
Leukocyte concentration (thou/μL) |
Leukocyte fold change |
Monocyte concentration (thou/μL) |
Lymphocyte concentration (thou/μL) |
Neutrophil concentration (thou/μL) |
|||
---|---|---|---|---|---|---|---|---|---|
Peripheral blood | 120 | 5.4 | 0.2 | 1.8 | 3.2 | ||||
PRP | Centrifugation factor |
||||||||
PRP layer | Top | 185 | 1.6 | 1.0 | 0.2 | 0.03 | 0.6 | 0.3 | |
Middle | 200 | 1.8 | 1.7 | 0.3 | 0.07 | 1.0 | 0.6 | ||
Bottom | 174* | 1.5* | 6.9*† | 1.2*† | 0.3*† | 2.8*† | 3.7*† | ||
Centrifugation Rate |
1,100 rpm (188 × g) |
186 | 1.6 | 3.9 | 0.7 | 0.2 | 1.8 | 1.8 | |
1300 rpm (263 × g) |
191 | 1.7 | 2.4 | 0.4 | 0.1 | 1.1 | 1.1 | ||
1,500 rpm (350 × g) |
181 | 1.6 | 3.4 | 0.6 | 0.2 | 1.4 | 1.7 | ||
Centrifugation braking |
(+) Brake |
189 | 1.7 | 3.9‡ | 0.7‡ | 0.2 | 1.7 | 1.8 | |
(–) Brake |
183 | 1.6 | 2.5‡ | 0.5‡ | 0.1 | 1.2 | 1.2 |
Concentrations represent the mean average of all samples (N = 4 horses for platelet concentration and fold change; N = 5 horses for Leukocyte, Monocyte, Lymphocyte, and Neutrophil concentration and Leukocyte fold change). Asterisk (*) represents a p < 0.05 between the bottom and middle layers of PRP; the dagger (†) represents a p < 0.05 between the top and bottom layers of PRP; and the double dagger (‡) represents a p < 0.05 between (+) and (–) centrifugation brake.