Skip to main content
. 2020 May 12;10:589. doi: 10.3389/fonc.2020.00589

Figure 1.

Figure 1

Downregulation of miR-221 induces autophagy in bladder cancer (BC) cells. (A) The expression of miR-221 was determined by RT-qPCR in BC cell lines. SV-HUC-1 was used as a normal control. (B) The Kaplan–Meier survival analysis of low and high level of miR-221 from The Cancer Genome Atlas (TCGA) database. (C) Relative expression of miR-221 after knockdown and overexpression of miR-221 examined by RT-qPCR. (D) T24 cells were transfected with miR-221 inhibitor or miR-NC inhibitor for 24 h, followed by exposure to 10 mM chloroquine (CQ). (E) Two representative transmission electron microscopy (TEM) images were identified to show the autophagosome after knockdown of miR-221 for 24 h. Red arrows indicate autolysosome. Scale bar, 1 μm. (F) T24 cells were transfected with miR-221 inhibitor for 48 h and then stained with Cyto-ID Autophagy Detection Kit (left) and LC3 antibody (right). Scale bar, 50 μm. *p < 0.05, ***p < 0.001, and ****p < 0.0001.